aBIOTECH

Table of Content

Volume 5 Issue 2,

Regulation of gene-edited plants in Europe: from the valley of tears into the shining sun?

Holger Puchta

A dual-function selection system enables positive selection of multigene CRISPR mutants and negative selection of Cas9-free progeny in Arabidopsis

Feng-Zhu Wang ...... Xiangyu Xiong & Jian-Feng Li

Integrating machine learning and genome editing for crop improvement

Long Chen, Guanqing Liu & Tao Zhang

The RUBY reporter for visual selection in soybean genome editing

Li Chen ...... Shuiqing Wu & Wensheng Hou

Fusion of a rice endogenous N-methylpurine DNA glycosylase to a plant adenine base transition editor ABE8e enables A-to-K base editing in rice plants

Yucai Li ...... Yan Guo & Lanqin Xia

Enhanced editing efficiency in Arabidopsis with a LbCas12a variant harboring D156R and E795L mutations

Cuiping Xin ...... Xue-Chen Wang & Qi-jun Chen

Consumer transparency in the production chain for plant varieties produced using new genomic techniques

J. M. Lukasiewicz ...... L. A. P. Lotz & M. J. M. Smulders

Expanding the targeting scope of CRISPR/Cas9-mediated genome editing by Cas9 variants in Brassica

Wenjing Li ...... Jianjun Zhao, Yan Li & Jun Li

Optimized protoplast isolation and transfection with a breakpoint: accelerating Cas9/sgRNA cleavage efficiency validation in monocot and dicot

Debasmita Panda ...... Mirza J. Baig & Kutubuddin A. Molla

Developing a CRISPR/FrCas9 system for core promoter editing in rice

Hui Wang ...... Pengcheng Wei & Juan Li

Efficient and precise genomic deletion in rice using enhanced prime editing

Mengyuan Liu ...... Jiuran Zhao & Jinxiao Yang

Exploiting viral vectors to deliver genome editing reagents in plants

Yilin Shen ...... Xiaoou Dong & Jianmin Wan

C-terminal frameshift mutations generate viable knockout mutants with developmental defects for three essential protein kinases

Yun Zhang ...... Yue-Dan Chen & Kabin Xie

Genome editing toward biofortified soybean with minimal trade-off between low phytic acid and yield

Wenxin Lin ...... Fanjiang Kong & Yuefeng Guan

Efficient genome editing in rice with miniature Cas12f variants

Zhengyan Ye ...... Junjie Tan & Jianmin Wan

Genome editing in plants using the TnpB transposase system

Qi Li ...... Wen Wang & Kang Duan

Simultaneous genetic transformation and genome editing of mixed lines in soybean (Glycine max) and maize (Zea mays)

Michelle Valentine ...... Thomas Ream & Yurong Chen